Journal: iScience

Article Title: The deubiquitinase Otud7b suppresses cone photoreceptor degeneration in mouse models of retinal degenerative diseases

doi: 10.1016/j.isci.2024.109380

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-S100β , Sigma , Cat#S2532; RRID: AB_477499.

Techniques: Plasmid Preparation, Recombinant, Imaging, Reporter Assay, Sequencing, shRNA, Software

Journal: Theranostics

Article Title: Visualizing Nerve Injury in a Neuropathic Pain Model with [ 18 F]FTC-146 PET/MRI

doi: 10.7150/thno.19378

Figure Lengend Snippet: Images and quantitation of ex vivo autoradiography and S1R staining of injured and uninjured rat sciatic nerve sections. ( A ) Representative autoradiography images of injured (upper panel) versus uninjured (lower panel) nerve sections, H&E staining of the same section used for autoradiography, and immunohistochemical staining of S1R in injured versus uninjured nerves. Whole nerve and muscle images were taken at 1× magnification, scale bar = 2.0 mm; neuroma images were taken at 40× magnification, scale bar = 50 µm. ( B ) Quantitative bar graphs showing the % area occupied by S1R staining and the mean pixel/S1R-staining intensity in injured (n = 4) versus uninjured (n = 4) nerves. Error bars represent standard error of the mean value, *p<0.05. ( C ) Double immunofluorescence staining of injured (two upper panels) and uninjured (two lower panels) sciatic nerves. Schwann cell (using anti-S100β antibody) is shown in green, whereas S1R immunostaining (using anti-S1R antibody) is shown in red. DAPI (4',6-diamidino-2-phenylindole) nuclear counterstaining is shown in blue, and the merged image of all three stains for both the injured and uninjured nerves is shown on the far right of each row. All images were taken at 40× magnification, scale bar = 10 µm.

Article Snippet: Briefly, we used rabbit polyclonal, affinity purified, anti-S1R antibody (1:100), and mouse monoclonal anti-S100β antibody (1:100, Sigma Aldrich, catalog number S2532), along with secondary antibodies 1:1000 (Alexa 488-conjugated goat anti-mouse IgG and Alexa 594-conjugated goat anti-rabbit IgG purchased from Jackson ImmunoResearch).

Techniques: Quantitation Assay, Ex Vivo, Autoradiography, Staining, Immunohistochemical staining, Double Immunofluorescence Staining, Immunostaining

Journal: bioRxiv

Article Title: Astrocyte-mediated transduction of muscle fiber contractions synchronizes hippocampal neuronal network development

doi: 10.1101/2022.04.12.487995

Figure Lengend Snippet: (A) Temporal change in synapse numbers between RM and CM on day 2 and 9. (B) Normalized vesicle accumulation per synapse between RM and CM on day 2 and 9. (C) Normalized F-actin intensity on day 9. (D) Temporal change in astrocyte numbers between RM and CM on day 2 and 9. (E) Confocal images of astrocytes in the cultures on day 2 in RM (left) and CM (right) (S100β, green; DAPI, blue). Scale bar: 50 μm. Data are represented as mean ± SEM (n = 6). Different lowercase letters indicate significant differences (p < 0.05).

Article Snippet: Primary antibodies were anti-synaptophysin monoclonal rabbit (1:1000; Abcam, ab32127), anti-PSD95 monoclonal mouse (1:1000; Invitrogen, MA1-046), anti-Bassoon monoclonal mouse (1:1000; Abcam, ab82958), anti-β III Tubulin polyclonal rabbit (1:1000; SYSY, 302 302), anti-S100β monoclonal mouse (1:1000; SYSY, 287 011), and Alexa Fluor 647-conjugated Phalloidin (1:500; Invitrogen, A22287).

Techniques: